PPCS recombinant monoclonal antibody, clone R01-6I3
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HeLa whole cell lysate and Lane 2: Jurkat whole cell lysate with PPCS recombinant monoclonal antibody, clone R01-6I3 (Cat # RAB04465).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PPCS.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PPCS.
Theoretical MW (kDa)
Calculated MW: 34 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, 150mM NaCl, pH 7.4 (40% glycerol, 0.05% BSA and 0.01% Sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HeLa whole cell lysate and Lane 2: Jurkat whole cell lysate with PPCS recombinant monoclonal antibody, clone R01-6I3 (Cat # RAB04465).Immunoprecipitation
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Gene Info — PPCS
Entrez GeneID
79717Protein Accession#
Q9HAB8Gene Name
PPCS
Gene Alias
FLJ11838, MGC117357, MGC138220
Gene Description
phosphopantothenoylcysteine synthetase
Omim ID
609853Gene Ontology
HyperlinkGene Summary
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM
Other Designations
OTTHUMP00000008431|OTTHUMP00000008433
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Interactome
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Pathway
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