UBE4B recombinant monoclonal antibody, clone R04-6C6
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: PC-12 whole cell lysate and Lane 2: C6 whole cell lysate with UBE4B recombinant monoclonal antibody, clone R04-6C6 (Cat # RAB04421).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human UBE4B.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human UBE4B.
Theoretical MW (kDa)
Calculated MW: 146 k
Reactivity
Human, Rat
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: PC-12 whole cell lysate and Lane 2: C6 whole cell lysate with UBE4B recombinant monoclonal antibody, clone R04-6C6 (Cat # RAB04421).Immunocytochemistry
Immunofluorescence
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Gene Info — UBE4B
Entrez GeneID
10277Protein Accession#
O95155Gene Name
UBE4B
Gene Alias
E4, HDNB1, KIAA0684, UBOX3, UFD2
Gene Description
ubiquitination factor E4B (UFD2 homolog, yeast)
Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000001726|OTTHUMP00000001727|homologous to yeast UFD2|homozygously deleted in neuroblastoma-1|ubiquitin-fusion degradation protein 2|ubiquitination factor E4B|ubiquitination factor E4B (homologous to yeast UFD2)
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Interactome
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Pathway
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Disease
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