CYP19A1 recombinant monoclonal antibody, clone 1H1
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate, Lane 2: Jurkat whole cell lysate, Lane 3: 293 whole cell lysate, Lane 4: SH-SY5Y whole cell lysate, Lane 5: U87 whole cell lysate and Lane 6: K562 whole cell lysate with CYP19A1 recombinant monoclonal antibody, clone 1H1 (Cat # RAB04357).
Immunohistochemistry
Immunohistochemical staining of human placenta tissue with CYP19A1 recombinant monoclonal antibody, clone 1H1 (Cat # RAB04357) (diluated at 1:230).
Immunofluorescence
Immunofluorescent staining of HepG2 cells with CYP19A1 recombinant monoclonal antibody, clone 1H1 (Cat # RAB04357) (diluated at 1:76). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CYP19A1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CYP19A1.
Theoretical MW (kDa)
Calculated MW: 58, 2
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate, Lane 2: Jurkat whole cell lysate, Lane 3: 293 whole cell lysate, Lane 4: SH-SY5Y whole cell lysate, Lane 5: U87 whole cell lysate and Lane 6: K562 whole cell lysate with CYP19A1 recombinant monoclonal antibody, clone 1H1 (Cat # RAB04357).Immunohistochemistry
Immunohistochemical staining of human placenta tissue with CYP19A1 recombinant monoclonal antibody, clone 1H1 (Cat # RAB04357) (diluated at 1:230).Immunofluorescence
Immunofluorescent staining of HepG2 cells with CYP19A1 recombinant monoclonal antibody, clone 1H1 (Cat # RAB04357) (diluated at 1:76). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CYP19A1
Entrez GeneID
1588Protein Accession#
P11511Gene Name
CYP19A1
Gene Alias
ARO, ARO1, CPV1, CYAR, CYP19, MGC104309, P-450AROM
Gene Description
cytochrome P450, family 19, subfamily A, polypeptide 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. The gene expresses two transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000162543|aromatase|cytochrome P450, family 19|cytochrome P450, subfamily XIX (aromatization of androgens)|estrogen synthetase|flavoprotein-linked monooxygenase|microsomal monooxygenase
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Interactome
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Pathway
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Disease
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