FEN1 recombinant monoclonal antibody, clone 4D9
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: Reji whole cell lysate, Lane 3: HepG2 whole cell lysate, Lane 4: Jurkat whole cell lysate and Lane 5: MCF-7 whole cell lysate with FEN1 recombinant monoclonal antibody, clone 4D9 (Cat # RAB04338).
Immunohistochemistry
Immunohistochemical staining of human small intestine tissue with FEN1 recombinant monoclonal antibody, clone 4D9 (Cat # RAB04338) (diluated at 1:77.5).
Immunofluorescence
Immunofluorescent staining of Hela cells with FEN1 recombinant monoclonal antibody, clone 4D9 (Cat # RAB04338) (diluated at 1:25). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human FEN1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human FEN1.
Theoretical MW (kDa)
Calculated MW: 43, 3
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: Reji whole cell lysate, Lane 3: HepG2 whole cell lysate, Lane 4: Jurkat whole cell lysate and Lane 5: MCF-7 whole cell lysate with FEN1 recombinant monoclonal antibody, clone 4D9 (Cat # RAB04338).Immunohistochemistry
Immunohistochemical staining of human small intestine tissue with FEN1 recombinant monoclonal antibody, clone 4D9 (Cat # RAB04338) (diluated at 1:77.5).Immunofluorescence
Immunofluorescent staining of Hela cells with FEN1 recombinant monoclonal antibody, clone 4D9 (Cat # RAB04338) (diluated at 1:25). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — FEN1
Entrez GeneID
2237Protein Accession#
P39748Gene Name
FEN1
Gene Alias
FEN-1, MF1, RAD2
Gene Description
flap structure-specific endonuclease 1
Omim ID
600393Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq
Other Designations
DNase IV|maturation factor-1
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Interactome
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