CDKN1B recombinant monoclonal antibody, clone 1G2
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate, Lane 2: Hela whole cell lysate, Lane 3: MCF-7 whole cell lysate and Lane 4: NIH/3T3 whole cell lysate with CDKN1B recombinant monoclonal antibody, clone 1G2 (Cat # RAB04334).
Immunohistochemistry
Immunohistochemical staining of human glioma cancer with CDKN1B recombinant monoclonal antibody, clone 1G2 (Cat # RAB04334) (diluated at 1:97.5).
Immunohistochemistry
Immunohistochemical staining of human tonsil tissue with CDKN1B recombinant monoclonal antibody, clone 1G2 (Cat # RAB04334) (diluated at 1:97.5).
Immunofluorescence
Immunofluorescent staining of HepG2 cells with CDKN1B recombinant monoclonal antibody, clone 1G2 (Cat # RAB04334) (diluated at 1:32.5). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CDKN1B.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CDKN1B.
Theoretical MW (kDa)
Calculated MW: 23 kD
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate, Lane 2: Hela whole cell lysate, Lane 3: MCF-7 whole cell lysate and Lane 4: NIH/3T3 whole cell lysate with CDKN1B recombinant monoclonal antibody, clone 1G2 (Cat # RAB04334).Immunohistochemistry
Immunohistochemical staining of human glioma cancer with CDKN1B recombinant monoclonal antibody, clone 1G2 (Cat # RAB04334) (diluated at 1:97.5).Immunohistochemistry
Immunohistochemical staining of human tonsil tissue with CDKN1B recombinant monoclonal antibody, clone 1G2 (Cat # RAB04334) (diluated at 1:97.5).Immunofluorescence
Immunofluorescent staining of HepG2 cells with CDKN1B recombinant monoclonal antibody, clone 1G2 (Cat # RAB04334) (diluated at 1:32.5). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
-
Gene Info — CDKN1B
Entrez GeneID
1027Protein Accession#
P46527Gene Name
CDKN1B
Gene Alias
CDKN4, KIP1, MEN1B, MEN4, P27KIP1
Gene Description
cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Gene Ontology
HyperlinkGene Summary
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq
Other Designations
cyclin-dependent kinase inhibitor 1B
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com