CCR9 recombinant monoclonal antibody, clone 4G2
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CCR9.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CCR9.
Theoretical MW (kDa)
Calculated MW: 43, 4
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: SH-SY5Y whole cell lysate, Lane 2: HepG2 whole cell lysate, Lane 3: Jurkat whole cell lysate, Lane 4: HL-60 whole cell lysate, Lane 5: Hela whole cell lysate, Lane 6: U87 whole cell lysate and Lane 7: mouse brain tissue with CCR9 recombinant monoclonal antibody, clone 4G2 (Cat # RAB04331).Immunofluorescence
Immunofluorescent staining of HepG2 cells with CCR9 recombinant monoclonal antibody, clone 4G2 (Cat # RAB04331) (diluated at 1:33). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of HL-60 cell lysate with CCR9 recombinant monoclonal antibody, clone 4G2 (Cat # RAB04331).
Lane 1: rabbit control IgG, Lane 2: RAB04331 precipitates and Lane 3: Input (HL-60 whole cell lysate).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CCR9
Entrez GeneID
10803Protein Accession#
P51686Gene Name
CCR9
Gene Alias
CDw199, GPR-9-6, GPR28
Gene Description
chemokine (C-C motif) receptor 9
Omim ID
604738Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
G protein-coupled receptor 28|OTTHUMP00000164653|OTTHUMP00000164654
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