CCR9 recombinant monoclonal antibody, clone 4G2

Catalog # RAB04331

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Price

Stock

Quantity

Size:100 uL
Price: USD $ 638.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot
Application

Western Blot

Western blot analysis of Lane 1: SH-SY5Y whole cell lysate, Lane 2: HepG2 whole cell lysate, Lane 3: Jurkat whole cell lysate, Lane 4: HL-60 whole cell lysate, Lane 5: Hela whole cell lysate, Lane 6: U87 whole cell lysate and Lane 7: mouse brain tissue with CCR9 recombinant monoclonal antibody, clone 4G2 (Cat # RAB04331).

Immunofluorescence
Application

Immunofluorescence

Immunofluorescent staining of HepG2 cells with CCR9 recombinant monoclonal antibody, clone 4G2 (Cat # RAB04331) (diluated at 1:33). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).

Immunoprecipitation
Application

Immunoprecipitation

Immunoprecipitation analysis of HL-60 cell lysate with CCR9 recombinant monoclonal antibody, clone 4G2 (Cat # RAB04331).
Lane 1: rabbit control IgG, Lane 2: RAB04331 precipitates and Lane 3: Input (HL-60 whole cell lysate).

  • Specification

    Product Description

    Rabbit recombinant monoclonal antibody raised against human CCR9.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against a synthetic peptide corresponding to human CCR9.

    Theoretical MW (kDa)

    Calculated MW: 43, 4

    Reactivity

    Human, Mouse

    Form

    Liquid

    Purification

    Affinity chromatography

    Isotype

    IgG

    Recommend Usage

    ELISA
    Immunofluorescence (1:20-1:200)
    Immunoprecipitation (1:200-1:1000)
    Western Blot (1:500-1:5000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)

    Storage Instruction

    Store at -20 °C or -80 °C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot

    Western blot analysis of Lane 1: SH-SY5Y whole cell lysate, Lane 2: HepG2 whole cell lysate, Lane 3: Jurkat whole cell lysate, Lane 4: HL-60 whole cell lysate, Lane 5: Hela whole cell lysate, Lane 6: U87 whole cell lysate and Lane 7: mouse brain tissue with CCR9 recombinant monoclonal antibody, clone 4G2 (Cat # RAB04331).

    Immunofluorescence

    Immunofluorescent staining of HepG2 cells with CCR9 recombinant monoclonal antibody, clone 4G2 (Cat # RAB04331) (diluated at 1:33). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).

    Immunoprecipitation

    Immunoprecipitation analysis of HL-60 cell lysate with CCR9 recombinant monoclonal antibody, clone 4G2 (Cat # RAB04331).
    Lane 1: rabbit control IgG, Lane 2: RAB04331 precipitates and Lane 3: Input (HL-60 whole cell lysate).

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — CCR9

    Entrez GeneID

    10803

    Protein Accession#

    P51686

    Gene Name

    CCR9

    Gene Alias

    CDw199, GPR-9-6, GPR28

    Gene Description

    chemokine (C-C motif) receptor 9

    Omim ID

    604738

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described. [provided by RefSeq

    Other Designations

    G protein-coupled receptor 28|OTTHUMP00000164653|OTTHUMP00000164654

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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