HNRNPK recombinant monoclonal antibody, clone 3G7
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: HepG2 whole cell lysate, Lane 4: 293T whole cell lysate, Lane 5: 293 whole cell lysate, Lane 6: U87 whole cell lysate and Lane 7: SH-SY5Y whole cell lysate with HNRNPK recombinant monoclonal antibody, clone 3G7 (Cat # RAB04328).
Immunohistochemistry
Immunohistochemical staining of human pancreatic cancer with HNRNPK recombinant monoclonal antibody, clone 3G7 (Cat # RAB04328) (diluated at 1:130.5).
Immunohistochemistry
Immunohistochemical staining of human cervical cancer with HNRNPK recombinant monoclonal antibody, clone 3G7 (Cat # RAB04328) (diluated at 1:130.5).
Immunofluorescence
Immunofluorescent staining of A549 cells with HNRNPK recombinant monoclonal antibody, clone 3G7 (Cat # RAB04328) (diluated at 1:43.5). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of HepG2 cell lysate with HNRNPK recombinant monoclonal antibody, clone 3G7 (Cat # RAB04328).
Lane 1: rabbit control IgG, Lane 2: RAB04328 precipitates and Lane 3: Input (HepG2 whole cell lysate).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HNRNPK.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HNRNPK.
Theoretical MW (kDa)
Calculated MW: 51, 5
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: HepG2 whole cell lysate, Lane 4: 293T whole cell lysate, Lane 5: 293 whole cell lysate, Lane 6: U87 whole cell lysate and Lane 7: SH-SY5Y whole cell lysate with HNRNPK recombinant monoclonal antibody, clone 3G7 (Cat # RAB04328).Immunohistochemistry
Immunohistochemical staining of human pancreatic cancer with HNRNPK recombinant monoclonal antibody, clone 3G7 (Cat # RAB04328) (diluated at 1:130.5).Immunohistochemistry
Immunohistochemical staining of human cervical cancer with HNRNPK recombinant monoclonal antibody, clone 3G7 (Cat # RAB04328) (diluated at 1:130.5).Immunofluorescence
Immunofluorescent staining of A549 cells with HNRNPK recombinant monoclonal antibody, clone 3G7 (Cat # RAB04328) (diluated at 1:43.5). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of HepG2 cell lysate with HNRNPK recombinant monoclonal antibody, clone 3G7 (Cat # RAB04328).
Lane 1: rabbit control IgG, Lane 2: RAB04328 precipitates and Lane 3: Input (HepG2 whole cell lysate).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HNRNPK
Entrez GeneID
3190Protein Accession#
P61978Gene Name
HNRNPK
Gene Alias
CSBP, FLJ41122, HNRPK, TUNP
Gene Description
heterogeneous nuclear ribonucleoprotein K
Omim ID
600712Gene Ontology
HyperlinkGene Summary
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq
Other Designations
OTTHUMP00000021554|OTTHUMP00000021557|OTTHUMP00000021558|dC-stretch binding protein|transformation upregulated nuclear protein
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Interactome
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