NDRG1 recombinant monoclonal antibody, clone 4C8
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NDRG1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human NDRG1.
Theoretical MW (kDa)
Calculated MW: 43, 3
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate, Lane 2: 293 whole cell lysate, Lane 3: PC3 whole cell lysate, Lane 4: U87 whole cell lysate and Lane 5: mouse kidney tissue with NDRG1 recombinant monoclonal antibody, clone 4C8 (Cat # RAB04315).Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with NDRG1 recombinant monoclonal antibody, clone 4C8 (Cat # RAB04315) (diluated at 1:71.6666666666667).Immunofluorescence
Immunofluorescent staining of Hela cells with NDRG1 recombinant monoclonal antibody, clone 4C8 (Cat # RAB04315) (diluated at 1:23). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — NDRG1
Entrez GeneID
10397Protein Accession#
Q92597Gene Name
NDRG1
Gene Alias
CAP43, CMT4D, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5
Gene Description
N-myc downstream regulated 1
Gene Ontology
HyperlinkGene Summary
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. It is necessary for p53-mediated caspase activation and apoptosis. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
differentiation-related gene 1 protein|nickel-specific induction protein Cap43|protein regulated by oxygen-1|reducing agents and tunicamycin-responsive protein|tunicamycin-responsive protein
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Interactome
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Disease
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