HDAC9 recombinant monoclonal antibody, clone 1F2
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate, Lane 2: MCK-7 whole cell lysate, Lane 3: 293T whole cell lysate and Lane 4: K562 whole cell lysate with HDAC9 recombinant monoclonal antibody, clone 1F2 (Cat # RAB04306).
Immunohistochemistry
Immunohistochemical staining of human kidney tissue with HDAC9 recombinant monoclonal antibody, clone 1F2 (Cat # RAB04306) (diluated at 1:154).
Immunohistochemistry
Immunohistochemical staining of human liver cancer with HDAC9 recombinant monoclonal antibody, clone 1F2 (Cat # RAB04306) (diluated at 1:154)
Immunofluorescence
Immunofluorescent staining of Hela cells with HDAC9 recombinant monoclonal antibody, clone 1F2 (Cat # RAB04306) (diluated at 1:51). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HDAC9.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HDAC9.
Theoretical MW (kDa)
Calculated MW: 112,
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1:Hela whole cell lysate, Lane 2: MCK-7 whole cell lysate, Lane 3: 293T whole cell lysate and Lane 4: K562 whole cell lysate with HDAC9 recombinant monoclonal antibody, clone 1F2 (Cat # RAB04306).Immunohistochemistry
Immunohistochemical staining of human kidney tissue with HDAC9 recombinant monoclonal antibody, clone 1F2 (Cat # RAB04306) (diluated at 1:154).Immunohistochemistry
Immunohistochemical staining of human liver cancer with HDAC9 recombinant monoclonal antibody, clone 1F2 (Cat # RAB04306) (diluated at 1:154)Immunofluorescence
Immunofluorescent staining of Hela cells with HDAC9 recombinant monoclonal antibody, clone 1F2 (Cat # RAB04306) (diluated at 1:51). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HDAC9
Entrez GeneID
9734Protein Accession#
Q9UKV0Gene Name
HDAC9
Gene Alias
DKFZp779K1053, HD7, HDAC, HDAC7, HDAC7B, HDAC9B, HDAC9FL, HDRP, KIAA0744, MITR
Gene Description
histone deacetylase 9
Omim ID
606543Gene Ontology
HyperlinkGene Summary
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined. [provided by RefSeq
Other Designations
MEF-2 interacting transcription repressor (MITR) protein|histone deacetylase 4/5-related protein|histone deacetylase 7|histone deacetylase 7B
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Interactome
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Disease
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