STAT3 (phospho S727) recombinant monoclonal antibody, clone 2A9
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: A549 whole cell lysate (treated with EGF 100 ng/mL/20 mins) and Lane 2: A549 whole cell lysate (not treated) with STAT3 (phospho S727) recombinant monoclonal antibody, clone 2A9 (Cat # RAB04251).
Immunohistochemistry
Immunohistochemical staining of human breast cancer with STAT3 (phospho S727) recombinant monoclonal antibody, clone 2A9 (Cat # RAB04251) (diluated at 1:100).
Immunoprecipitation
Immunoprecipitation analysis of 293 cell lysate with STAT3 (phospho S727) recombinant monoclonal antibody, clone 2A9 (Cat # RAB04251).
Lane 1: rabbit control IgG, Lane 2: RAB04251 precipitates and Lane 3 : Input (293 whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human STAT3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S727 of human STAT3.
Theoretical MW (kDa)
Calculated MW: 98 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: A549 whole cell lysate (treated with EGF 100 ng/mL/20 mins) and Lane 2: A549 whole cell lysate (not treated) with STAT3 (phospho S727) recombinant monoclonal antibody, clone 2A9 (Cat # RAB04251).Immunohistochemistry
Immunohistochemical staining of human breast cancer with STAT3 (phospho S727) recombinant monoclonal antibody, clone 2A9 (Cat # RAB04251) (diluated at 1:100).Immunoprecipitation
Immunoprecipitation analysis of 293 cell lysate with STAT3 (phospho S727) recombinant monoclonal antibody, clone 2A9 (Cat # RAB04251).
Lane 1: rabbit control IgG, Lane 2: RAB04251 precipitates and Lane 3 : Input (293 whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — STAT3
Entrez GeneID
6774Protein Accession#
P40763Gene Name
STAT3
Gene Alias
APRF, FLJ20882, HIES, MGC16063
Gene Description
signal transducer and activator of transcription 3 (acute-phase response factor)
Omim ID
102582Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
DNA-binding protein APRF|acute-phase response factor|signal transducer and activator of transcription 3
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Interactome
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Disease
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