CD81 recombinant monoclonal antibody, clone 9F7
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD81.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CD81.
Theoretical MW (kDa)
Calculated MW: 26 kD
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry (1:50-1:500)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of K562 whole cell lysate with CD81 recombinant monoclonal antibody, clone 9F7 (Cat # RAB04209).Immunohistochemistry
Immunohistochemical staining of human testis tissue with CD81 recombinant monoclonal antibody, clone 9F7 (Cat # RAB04209) (diluated at 1:100).Immunohistochemistry
Immunohistochemical staining of human tonsil tissue with CD81 recombinant monoclonal antibody, clone 9F7 (Cat # RAB04209) (diluated at 1:100).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells with CD81 recombinant monoclonal antibody, clone 9F7 (Cat # RAB04209) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — CD81
Entrez GeneID
975Protein Accession#
P60033Gene Name
CD81
Gene Alias
S5.7, TAPA1, TSPAN28
Gene Description
CD81 molecule
Omim ID
186845Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq
Other Designations
26 kDa cell surface protein TAPA-1|CD81 antigen|CD81 antigen (target of antiproliferative antibody 1)|target of antiproliferative antibody 1
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