CR2 recombinant monoclonal antibody, clone 16F10
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CR2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CR2.
Theoretical MW (kDa)
Calculated MW: 155 k
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:30-1:200)
Immunohistochemistry (1:50-1:500)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Raji whole cell lysate with CR2 recombinant monoclonal antibody, clone 16F10 (Cat # RAB04206).Immunohistochemistry
Immunohistochemical staining of human lung cancer with CR2 recombinant monoclonal antibody, clone 16F10 (Cat # RAB04206) (diluated at 1:100)Immunohistochemistry
Immunohistochemical staining of human tonsil tissue with CR2 recombinant monoclonal antibody, clone 16F10 (Cat # RAB04206) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of Raji cells with CR2 recombinant monoclonal antibody, clone 16F10 (Cat # RAB04206) (diluated at 1:34). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Raji cells with CR2 recombinant monoclonal antibody, clone 16F10 (Cat # RAB04206) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — CR2
Entrez GeneID
1380Protein Accession#
P20023Gene Name
CR2
Gene Alias
C3DR, CD21, SLEB9
Gene Description
complement component (3d/Epstein Barr virus) receptor 2
Gene Ontology
HyperlinkGene Summary
Complement component receptor-2 (CR2) is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. See also CR1 (MIM 120620). Yefenof et al. (1976) [PubMed 181330] found complete overlapping of EBV receptors and C3 (MIM 120700) receptors on human B lymphocytes.[supplied by OMIM
Other Designations
OTTHUMP00000034421|OTTHUMP00000034447
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