Histone H4 (acetyl K5) recombinant monoclonal antibody, clone 2C9
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More Files
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Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: 293 whole cell lysateand and Lane 4: HepG2 whole cell lysatewith Histone H4 (acetyl K5) recombinant monoclonal antibody, clone 2C9 (Cat # RAB04181).
Immunocytochemistry
Immunocytochemical staining of HeLa cells with Histone H4 (acetyl K5) recombinant monoclonal antibody, clone 2C9 (Cat # RAB04181) (dluated at 1:100).
Immunofluorescence
Immunofluorescent staining of Hela cells with Histone H4 (acetyl K5) recombinant monoclonal antibody, clone 2C9 (Cat # RAB04181) (diluated at 1:65). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human histone H4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic acetylated peptide corresponding to residues surrounding K5 of human histone H4.
Theoretical MW (kDa)
Calculated MW: 11 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunocytochemistry (1:50-1:500)
Immunofluorescence (1:30-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela whole cell lysate, Lane 2: MCF-7 whole cell lysate, Lane 3: 293 whole cell lysateand and Lane 4: HepG2 whole cell lysatewith Histone H4 (acetyl K5) recombinant monoclonal antibody, clone 2C9 (Cat # RAB04181).Immunocytochemistry
Immunocytochemical staining of HeLa cells with Histone H4 (acetyl K5) recombinant monoclonal antibody, clone 2C9 (Cat # RAB04181) (dluated at 1:100).Immunofluorescence
Immunofluorescent staining of Hela cells with Histone H4 (acetyl K5) recombinant monoclonal antibody, clone 2C9 (Cat # RAB04181) (diluated at 1:65). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HIST1H4A
Entrez GeneID
8359Protein Accession#
P62805Gene Name
HIST1H4A
Gene Alias
H4/a, H4FA
Gene Description
histone cluster 1, H4a
Omim ID
602822Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H4 histone family, member A|histone 1, H4a
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