CD47 recombinant monoclonal antibody, clone 4G11
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: A549 whole cell lysate; Lane 3: HepG2 whole cell lysate.
Immunohistochemistry
Immunohistochemistry image of CD47 recombinant monoclonal antibody, clone 4G11 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of MCF7 Cells with CD47 recombinant monoclonal antibody, clone 4G11 at 1:50, counter-stained with DAPI.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD47.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human CD47.
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunofluorescence(1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: A549 whole cell lysate; Lane 3: HepG2 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of CD47 recombinant monoclonal antibody, clone 4G11 diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of MCF7 Cells with CD47 recombinant monoclonal antibody, clone 4G11 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CD47
Entrez GeneID
961Protein Accession#
Q08722Gene Name
CD47
Gene Alias
IAP, MER6, OA3
Gene Description
CD47 molecule
Omim ID
601028Gene Ontology
HyperlinkGene Summary
This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD47 antigen|CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|CD47 glycoprotein|Rh-related antigen|antigen identified by monoclonal antibody 1D8|antigenic surface determinant protein OA3|integrin associated protein|integrin-associa
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Interactome
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Pathway
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