MAD2L2 recombinant monoclonal antibody, clone 4D8
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: 293 whole cell lysate.
Immunohistochemistry
Immunohistochemistry image of MAD2L2 recombinant monoclonal antibody, clone 4D8 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of Hela Cells with MAD2L2 recombinant monoclonal antibody, clone 4D8 at 1:50, counter-stained with DAPI.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human MAD2L2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human MAD2L2.
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunofluorescence(1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: 293 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of MAD2L2 recombinant monoclonal antibody, clone 4D8 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of Hela Cells with MAD2L2 recombinant monoclonal antibody, clone 4D8 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAD2L2
Entrez GeneID
10459Protein Accession#
Q9UI95Gene Name
MAD2L2
Gene Alias
MAD2B, REV7
Gene Description
MAD2 mitotic arrest deficient-like 2 (yeast)
Omim ID
604094Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins. [provided by RefSeq
Other Designations
MAD2 (mitotic arrest deficient, yeast, homolog)-like 2|MAD2 homolog|OTTHUMP00000002273|OTTHUMP00000002275|mitotic arrest deficient homolog-like 2
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Interactome
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Pathway
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Disease
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