DHFR recombinant monoclonal antibody, clone 9B2
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More Files
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human, mouse and rat DHFR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human DHFR.
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunoprecipitation(1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: Jurkat whole cell lysate; Lane 3: K562 whole cell lysate; Lane 4: Raji whole cell lysate; Lane 5: 293T whole cell lysate; Lane 6: Rat heart tissue; Lane 7: Mouse kidney tissue; Lane 8: Mouse liver tissue.Immunohistochemistry
Immunohistochemistry image of DHFR recombinant monoclonal antibody, clone 9B2 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system.Immunoprecipitation
Immunoprecipitating DHFR in Hela whole cell lysate.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DHFR
Entrez GeneID
1719Protein Accession#
P00374Gene Name
DHFR
Gene Alias
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Gene Description
dihydrofolate reductase
Omim ID
126060Gene Ontology
HyperlinkGene Summary
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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