ATP5A1 recombinant monoclonal antibody, clone 5G11
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human andd mouse ATP5A1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human ATP5A1.
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: HepG2 whole cell lysate; Lane 2: MCF-7 whole cell lysate; Lane 3: 293 whole cell lysate; Lane 4: HL60 whole cell lysate; Lane 5: Mouse Heart tissue; Lane 6: Mouse brain tissue.Immunohistochemistry
Immunohistochemistry image of ATP5A1 recombinant monoclonal antibody, clone 5G11 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ATP5A1
Entrez GeneID
498Protein Accession#
P25705Gene Name
ATP5A1
Gene Alias
ATP5A, ATP5AL2, ATPM, MOM2, OMR, ORM, hATP1
Gene Description
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Omim ID
164360Gene Ontology
HyperlinkGene Summary
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq
Other Designations
ATP synthase alpha chain, mitochondrial|ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit|ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle|ATP synthase, H+ transporting, mitochondrial F
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