PIK3CA recombinant monoclonal antibody, clone 10E5
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Jurkat whole cell lysate; Lane 2: HepG2 whole cell lysate; Lane 3: Hela whole cell lysate; Lane 4: NIH/3T3 whole cell lysate; Lane 5: K562 whole cell lysate; Lane 6: 293 whole cell lysate; Lane 7: MCF-7 whole cell lysate.
Immunofluorescence
Immunofluorescence staining of Hela Cells with PIK3CA recombinant monoclonal antibody, clone 10E5 at 1:50, counter-stained with DAPI.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and mouse PIK3CA.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human PIK3CA.
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence(1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Jurkat whole cell lysate; Lane 2: HepG2 whole cell lysate; Lane 3: Hela whole cell lysate; Lane 4: NIH/3T3 whole cell lysate; Lane 5: K562 whole cell lysate; Lane 6: 293 whole cell lysate; Lane 7: MCF-7 whole cell lysate.Immunofluorescence
Immunofluorescence staining of Hela Cells with PIK3CA recombinant monoclonal antibody, clone 10E5 at 1:50, counter-stained with DAPI.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PIK3CA
Entrez GeneID
5290Protein Accession#
P42336Gene Name
PIK3CA
Gene Alias
MGC142161, MGC142163, PI3K, p110-alpha
Gene Description
phosphoinositide-3-kinase, catalytic, alpha polypeptide
Gene Ontology
HyperlinkGene Summary
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq
Other Designations
PI3-kinase p110 subunit alpha|PtdIns-3-kinase p110|phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha|phosphatidylinositol 3-kinase, catalytic, alpha polypeptide|phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
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Interactome
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Pathway
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Disease
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