TRAF2 recombinant monoclonal antibody, clone 9A5
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: HepG2 whole cell lysate; Lane 2: Hela whole cell lysate; Lane 3: 293T whole cell lysate; Lane 4: Jurkat whole cell lysate; Lane 5: Raji whole cell lysate; Lane 6: K562 whole cell lysate.
Immunohistochemistry
Immunohistochemistry image of TRAF2 recombinant monoclonal antibody, clone 9A5 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system.
Immunoprecipitation
Immunoprecipitating TRAF2 in Hela whole cell lysate.
Flow Cytometry
Overlay histogram showing Hela cells stained with TRAF2 recombinant monoclonal antibody, clone 9A5 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human TRAF2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human TRAF2.
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation(1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: HepG2 whole cell lysate; Lane 2: Hela whole cell lysate; Lane 3: 293T whole cell lysate; Lane 4: Jurkat whole cell lysate; Lane 5: Raji whole cell lysate; Lane 6: K562 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of TRAF2 recombinant monoclonal antibody, clone 9A5 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system.Immunoprecipitation
Immunoprecipitating TRAF2 in Hela whole cell lysate.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay histogram showing Hela cells stained with TRAF2 recombinant monoclonal antibody, clone 9A5 (red line) at 1:50. -
Gene Info — TRAF2
Entrez GeneID
7186Protein Accession#
Q12933Gene Name
TRAF2
Gene Alias
MGC:45012, TRAP, TRAP3
Gene Description
TNF receptor-associated factor 2
Omim ID
601895Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined. [provided by RefSeq
Other Designations
OTTHUMP00000022625|OTTHUMP00000064745|tumor necrosis factor type 2 receptor associated protein 3
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