CDK4 recombinant monoclonal antibody, clone 8F2
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: HepG2 whole cell lysate; Lane 3: Jurkat whole cell lysate; Lane 4: 293 whole cell lysate; Lane 5: MCF-7 whole cell lysate; Lane 6: PC-3 whole cell lysate.
Immunoprecipitation
Immunoprecipitating CDK4 in Hela whole cell lysate.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CDK4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human CDK4.
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunoprecipitation(1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: HepG2 whole cell lysate; Lane 3: Jurkat whole cell lysate; Lane 4: 293 whole cell lysate; Lane 5: MCF-7 whole cell lysate; Lane 6: PC-3 whole cell lysate.Immunoprecipitation
Immunoprecipitating CDK4 in Hela whole cell lysate.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDK4
Entrez GeneID
1019Protein Accession#
P11802Gene Name
CDK4
Gene Alias
CMM3, MGC14458, PSK-J3
Gene Description
cyclin-dependent kinase 4
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq
Other Designations
cell division kinase 4|melanoma cutaneous malignant, 3
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Interactome
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Pathway
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Disease
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