PKM recombinant monoclonal antibody, clone 7B2
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: SH-SY5Y whole cell lysate; Lane 2: Jurkat whole cell lysate; Lane 3: MCF-7 whole cell lysate; Lane 4: Hela whole cell lysate; Lane 5: Raji whole cell lysate; Lane 6: 293 whole cell lysate; Lane 7: Mouse brain tissue.
Immunohistochemistry
Immunohistochemistry image of PKM recombinant monoclonal antibody, clone 7B2 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system.
Immunofluorescence
Immunofluorescence staining of Hela Cells with PKM recombinant monoclonal antibody, clone 7B2 at 1:50, counter-stained with DAPI.
Immunoprecipitation
Immunoprecipitating PKM in Hela whole cell lysate.
Flow Cytometry
Overlay histogram showing HepG2 cells stained with PKM recombinant monoclonal antibody, clone 7B2 (red line) at 1:50.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and mouse PKM.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human PKM.
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunofluorescence(1:20-1:200)
Immunoprecipitation(1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: SH-SY5Y whole cell lysate; Lane 2: Jurkat whole cell lysate; Lane 3: MCF-7 whole cell lysate; Lane 4: Hela whole cell lysate; Lane 5: Raji whole cell lysate; Lane 6: 293 whole cell lysate; Lane 7: Mouse brain tissue.Immunohistochemistry
Immunohistochemistry image of PKM recombinant monoclonal antibody, clone 7B2 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system.Immunofluorescence
Immunofluorescence staining of Hela Cells with PKM recombinant monoclonal antibody, clone 7B2 at 1:50, counter-stained with DAPI.Immunoprecipitation
Immunoprecipitating PKM in Hela whole cell lysate.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Overlay histogram showing HepG2 cells stained with PKM recombinant monoclonal antibody, clone 7B2 (red line) at 1:50. -
Gene Info — PKM2
Entrez GeneID
5315Protein Accession#
P14618Gene Name
PKM2
Gene Alias
CTHBP, MGC3932, OIP3, PK3, PKM, TCB, THBP1
Gene Description
pyruvate kinase, muscle
Omim ID
179050Gene Ontology
HyperlinkGene Summary
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq
Other Designations
OPA-interacting protein 3|PK, muscle type|pyruvate kinase M2|thyroid hormone-binding protein, cytosolic
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Glycolysis / Gluconeogenesis
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