MUC1 recombinant monoclonal antibody, clone Mc5
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More Files
- More Functions
Western Blot
Western blot analysis of MUC1 in MCF-7 cell lysates using human anti-Breast epithelial mucin recombinant monoclonal antibody, clone Mc5 (Cat # RAB02788).
Immunofluorescence
Immunofluorescence analysis of MUC1 (green) in MCF-7 cell lysates using human anti-Breast epithelial mucin recombinant monoclonal antibody, clone Mc5 (Cat # RAB02788), and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human breast epithelial mucin.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human breast epithelial mucin
Reactivity
Human
Form
Liquid
Conjugation
Unconjugated
Concentration
batch dependent
Isotype
IgG kappa
Recommend Usage
ELISA
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
IRA
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of MUC1 in MCF-7 cell lysates using human anti-Breast epithelial mucin recombinant monoclonal antibody, clone Mc5 (Cat # RAB02788).Immunohistochemistry
Immunofluorescence
Immunofluorescence analysis of MUC1 (green) in MCF-7 cell lysates using human anti-Breast epithelial mucin recombinant monoclonal antibody, clone Mc5 (Cat # RAB02788), and DAPI(blue).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MUC1
Entrez GeneID
4582Protein Accession#
P15941Gene Name
MUC1
Gene Alias
CD227, EMA, H23AG, MAM6, PEM, PEMT, PUM
Gene Description
mucin 1, cell surface associated
Omim ID
158340Gene Ontology
HyperlinkGene Summary
This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined. [provided by RefSeq
Other Designations
DF3 antigen|H23 antigen|MUC-1/SEC|MUC-1/X|MUC1/ZD|OTTHUMP00000033787|OTTHUMP00000033790|OTTHUMP00000033795|breast carcinoma-associated antigen DF3|episialin|mucin 1|mucin 1, transmembrane|peanut-reactive urinary mucin|polymorphic epithelial mucin|tumor as
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Interactome
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Disease
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