ANXA4 recombinant monoclonal antibody, clone SAIC-14C-10F12
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HepG2, Lane 2: A431 and Lane 3: HeLa lysates with ANXA4 recombinant monoclonal antibody, clone SAIC-14C-10F12 (Cat # RAB03065).
Immunofluorescence
Immunofluorescet staining of HepG2 cells with ANXA4 recombinant monoclonal antibody, clone SAIC-14C-10F12 (Cat # RAB03065).
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells permewith 0.15% Triton stained with the chimeric r version of RAB03065 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03065
(B) DAPI
(C) Merged channels
(D) Isotype control
Flow Cytometry
Flow cytometric analysis of HepG2 cells with ANXA4 recombinant monoclonal antibody, clone SAIC-14C-10F12 (Cat # RAB03065).
Paraformaldehyde fixed HepG2 cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03065 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ANXA1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide "DEGNYLDDALVR" corresponding to human ANXA4.
Reactivity
Human
Form
Liquid
Conjugation
Unconjugated
Concentration
batch dependent
Isotype
IgG kappa
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence
Immuno-MRM (multiple reaction monitoring)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of Lane 1: HepG2, Lane 2: A431 and Lane 3: HeLa lysates with ANXA4 recombinant monoclonal antibody, clone SAIC-14C-10F12 (Cat # RAB03065).Immunofluorescence
Immunofluorescet staining of HepG2 cells with ANXA4 recombinant monoclonal antibody, clone SAIC-14C-10F12 (Cat # RAB03065).
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells permewith 0.15% Triton stained with the chimeric r version of RAB03065 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03065
(B) DAPI
(C) Merged channels
(D) Isotype controlEnzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HepG2 cells with ANXA4 recombinant monoclonal antibody, clone SAIC-14C-10F12 (Cat # RAB03065).
Paraformaldehyde fixed HepG2 cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03065 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Immuno-MRM (multiple reaction monitoring)
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Gene Info — ANXA4
Entrez GeneID
307Gene Name
ANXA4
Gene Alias
ANX4, DKFZp686H02120, MGC75105, PIG28, ZAP36
Gene Description
annexin A4
Omim ID
106491Gene Ontology
HyperlinkGene Summary
Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. [provided by RefSeq
Other Designations
annexin IV|annexin IV (placental anticoagulant protein II)|placental anticoagulant protein II|proliferation-inducing gene 28|proliferation-inducing protein 28
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Interactome
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Disease
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