Tnfsf9 recombinant monoclonal antibody, clone AT113-2
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More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Tnfsf9 recombinant monoclonal antibody, clone AT113-2 (Cat # RAB03049).
Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03049 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03049
(B) DAPI
(C) Merged channels
(D) Isotype control
Flow Cytometry
Flow cytometric analysis of RAW264.7 cells with Tnfsf9 recombinant monoclonal antibody, clone AT113-2 (Cat # RAB03049).
Paraformaldehyde fixed RAW264.7 cells were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03345 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Tnfsf9.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against soluble recombinant Tnfsf9:Fc fusion protein.
Reactivity
Mouse
Form
Liquid
Conjugation
Unconjugated
Concentration
batch dependent
Isotype
IgG kappa
Recommend Usage
Blocking
Flow Cytometry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Tnfsf9 recombinant monoclonal antibody, clone AT113-2 (Cat # RAB03049).
Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03049 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03049
(B) DAPI
(C) Merged channels
(D) Isotype controlBlocking
Flow Cytometry
Flow cytometric analysis of RAW264.7 cells with Tnfsf9 recombinant monoclonal antibody, clone AT113-2 (Cat # RAB03049).
Paraformaldehyde fixed RAW264.7 cells were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03345 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — Tnfsf9
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