Products

Western Blot

Western blot analysis of Lane 1: A549, Lane 2: HepG2 and Lane 3: Hela cell lysates with MMP14 recombinant monoclonal antibody, clone LEM-2/15 (Cat # RAB03640).
Western Blot analysis the cell lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of RAB03640 at 0.3 ug/mL before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescence

Immunofluorescent staining of Hela cells with MMP14 recombinant monoclonal antibody, clone LEM-2/15 (Cat # RAB03640).
Immunofluorescence analysis of paraformaldehyde fixed Hela cells on Shi-fix™ coverslips permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of RAB03640 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing nuclear membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.

Enzyme-linked Immunoabsorbent Assay

Blocking

Entrez GeneID

4323

Gene Name

MMP14

Gene Alias

MMP-X1, MT1-MMP, MTMMP1

Gene Description

matrix metallopeptidase 14 (membrane-inserted)

Omim ID

600754

Gene Ontology

Hyperlink

Gene Summary

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq

Other Designations

matrix metalloproteinase 14|matrix metalloproteinase 14 (membrane-inserted)|membrane type 1 metalloprotease|membrane-type matrix metalloproteinase 1|membrane-type-1 matrix metalloproteinase