L3MBTL1 recombinant monoclonal antibody, clone RAB-C213
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More Files
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Immunofluorescence
Immunofluorescent staining of Kelly cells with L3MBTL1 recombinant monoclonal antibody, clone RAB-C213 (Cat # RAB03619).
Immunofluorescence analysis of paraformaldehyde fixed Kelly cells permeabilized with 0.15% Triton stained with the chimeric r version of RAB03619 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1000, showing cytoplasmic staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03619
(B) DAPI
(C) Merged channels
(D) Isotype control
Flow Cytometry
Flow cytometric analysis of Kelly cells with L3MBTL1 recombinant monoclonal antibody, clone RAB-C213 (Cat # RAB03619).
The fixed Kelly cells were permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03619 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human L3MBTL1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against L3MBTL1 protein under non-denaturing conditions.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
ChIP
ELISA
Flow Cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
ChIP
Immunofluorescence
Immunofluorescent staining of Kelly cells with L3MBTL1 recombinant monoclonal antibody, clone RAB-C213 (Cat # RAB03619).
Immunofluorescence analysis of paraformaldehyde fixed Kelly cells permeabilized with 0.15% Triton stained with the chimeric r version of RAB03619 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1000, showing cytoplasmic staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03619
(B) DAPI
(C) Merged channels
(D) Isotype controlEnzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Kelly cells with L3MBTL1 recombinant monoclonal antibody, clone RAB-C213 (Cat # RAB03619).
The fixed Kelly cells were permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03619 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — L3MBTL
Entrez GeneID
26013Gene Name
L3MBTL
Gene Alias
DKFZp586P1522, FLJ41181, H-L(3)MBT, KIAA0681, L3MBTL1, dJ138B7.3
Gene Description
l(3)mbt-like (Drosophila)
Omim ID
608802Gene Ontology
HyperlinkGene Summary
This gene encodes the homolog of a protein identified in Drosophila as a suppressor of malignant transformation of neuroblasts and ganglion-mother cells in the optic centers of the brain. This gene product is localized to condensed chromosomes in mitotic cells. Overexpression of this gene in a glioma cell line results in improper nuclear segregation and cytokinesis producing multinucleated cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000031024|l(3)mbt-like|lethal (3) malignant brain tumor l(3)
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