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Immunofluorescence

Immunofluorescent staining of human peripheral blood leukocytes (PBL) with CXCR2 recombinant monoclonal antibody, clone HC2 (Cat # RAB03596).
Immunofluorescence analysis of paraformaldehyde fixed human PBL cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03596 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03596
(B) DAPI
(C) Merged channels
(D) Isotype control

Flow Cytometry

Flow cytometric analysis of human peripheral blood monocytes (PBM) with CXCR2 recombinant monoclonal antibody, clone HC2 (Cat # RAB03596).
Paraformaldehyde fixed human PBM cells were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03596 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Entrez GeneID

3579

Gene Name

CXCR2

Gene Alias

CD182, CDw128b, CMKAR2, IL8R2, IL8RA, IL8RB

Gene Description

chemokine (C-X-C motif) receptor 2

Omim ID

146928

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. [provided by RefSeq

Other Designations

C-X-C chemokine receptor type 2|CXC-R2|CXCR-2|CXCR2 gene for IL8 receptor type B|GRO/MGSA receptor|IL-8 receptor type 2|IL-8R B|chemokine (CXC) receptor 2|high affinity interleukin-8 receptor B|interleukin 8 receptor B|interleukin 8 receptor type 2|interl