IFNG recombinant monoclonal antibody, clone D9D10
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More Files
- More Functions
Western Blot
Western blot analysis of human thymus lysate with IFNG recombinant monoclonal antibody, clone D9D10 (Cat # RAB03552).
Western Blot analysis the lysates (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots were probed with the chimeric r version of RAB03552 at 0.1 ug/mL before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human IFNG.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against FPLC-purified natural human IFNG.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Blocking
ELISA
Flow Cytometry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of human thymus lysate with IFNG recombinant monoclonal antibody, clone D9D10 (Cat # RAB03552).
Western Blot analysis the lysates (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots were probed with the chimeric r version of RAB03552 at 0.1 ug/mL before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
Blocking
Flow Cytometry
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Gene Info — IFNG
Entrez GeneID
3458Gene Name
IFNG
Gene Alias
IFG, IFI
Gene Description
interferon, gamma
Gene Ontology
HyperlinkGene Summary
Interferon-gamma (IFNG), or type II interferon, is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFNG in the immune system stems in part from its ability to inhibit viral replication directly, but most importantly derives from its immunostimulatory and immunomodulatory effects. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 (MIM 186940) and CD8 (see MIM 186910) cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops (Schoenborn and Wilson, 2007 [PubMed 17981204]).[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Disease
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