GCGR recombinant monoclonal antibody, clone hGR-2 F6
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More Files
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Immunofluorescence
Immunofluorescent staining of HepG2 cells with GCGR recombinant monoclonal antibody, clone hGR-2 F6 (Cat # RAB03546).
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of RAB03546 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing punctate membrane staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against human GCGR.
Antibody Species
Mouse
Immunogen
Original antibody is raised against recombinant protein corresponding to human GCGR.
Reactivity
Human
Form
Liquid
Isotype
IgG1 kappa
Recommend Usage
Blocking
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of HepG2 cells with GCGR recombinant monoclonal antibody, clone hGR-2 F6 (Cat # RAB03546).
Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of RAB03546 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing punctate membrane staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.Blocking
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Gene Info — GCGR
Entrez GeneID
2642Gene Name
GCGR
Gene Alias
GGR, MGC138246
Gene Description
glucagon receptor
Gene Ontology
HyperlinkGene Summary
The physiologic effects of glucagon (GCG; MIM 138030) are mediated through the glucagon receptor, a member of the superfamily of receptors characterized by a 7-transmembrane domain structure and by their coupling via GTP-binding proteins (G proteins) to adenyl cyclase.[supplied by OMIM
Other Designations
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Interactome
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Pathway
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Disease
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