GYPA recombinant monoclonal antibody, clone M2A1
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against human GYPA.
Antibody Species
Mouse
Immunogen
Original antibody is raised against human red blood cells of the M phenotype prepared by immunzing BALB/c mice.
Reactivity
Human
Form
Liquid
Isotype
IgG1 kappa
Recommend Usage
ELISA
Inhibition Assay
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescenct staining of K562 cells with GYPA recombinant monoclonal antibody, clone M2A1 (Cat # RAB03493).
Immunofluorescence analysis of paraformaldehyde fixed K562 cells permeabilized with 0.15% Triton stained with the chimeric mouse IgG1 version of RAB03493 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.Enzyme-linked Immunoabsorbent Assay
Inhibition Assay
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Gene Info — GYPA
Entrez GeneID
2993Gene Name
GYPA
Gene Alias
CD235a, GPA, GPErik, GPSAT, GpMiIII, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MNS
Gene Description
glycophorin A (MNS blood group)
Gene Ontology
HyperlinkGene Summary
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq
Other Designations
Mi.V glycoprotein (24 AA)|erythroid-lineage-specific membrane sialoglycoprotein|glycophorin A|glycophorin A (MN blood group)|glycophorin A MNS blood group|glycophorin A, GPA|glycophorin Erik|glycophorin MiI|glycophorin MiIII|glycophorin MiV|glycophorin Mi
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Disease
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