Products

Western Blot

Western blot analysis of Jurkat cell (35ug protein in RIPA buffer) with CLEC4M recombinant monoclonal antibody (0.1 ug/mL), clone 16E7 (Cat # RAB03406). A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunohistochemistry

Immunofluorescence

Immunofluorescent staining of Hela cells with CLEC4M recombinant monoclonal antibody, clone 16E7 (Cat # RAB03406).
Immunofluorescence analysis of paraformaldehyde Hela cells fixed on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric r version of RAB03406 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). The negative control was stained with unimmunized r followed by Alexa Fluor® 488 secondary antibody.

Flow Cytometry

Entrez GeneID

10332

Gene Name

CLEC4M

Gene Alias

CD209L, CD299, DC-SIGN2, DC-SIGNR, DCSIGNR, HP10347, L-SIGN, LSIGN, MGC129964, MGC47866

Gene Description

C-type lectin domain family 4, member M

Omim ID

605872

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a transmembrane receptor and is often referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses, with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are common and have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 30835; often referred to as DC-SIGN or CD209). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants

Other Designations

CD209 antigen-like|CD299 antigen|dendritic cell-specific ICAM-3-grabbing nonintegrin 2|liver/lymph node-specific ICAM-3 grabbing non-integrin|mannose binding C-type lectin DC-SIGNR