CLEC4A recombinant monoclonal antibody, clone 9E8
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More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of human peripheral blood monocytes with anti-DCIR antibody, clone 9E8 (Cat # RAB03404).
Immunofluorescence analysis of paraformaldehyde human peripheral blood monocytes fixed on Shi-fix™ coverslips, stained with the chimeric r version of RAB03404 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/mL), showing cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). The isotype control was stained with an anti-Fluorescein antibody.
Flow Cytometry
Flow cytometric analysis of human peripheral blood leukocytes with CLEC4A recombinant monoclonal antibody, clone 9E8 (Cat # RAB03404).
Paraformaldehyde fixed Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (isotype control-black line) or the r1 version of RAB03404 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CLEC4A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a dendritic cell immunoreceptor ectodomain-human Fc fusion protein.
Reactivity
Human
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Flow Cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of human peripheral blood monocytes with anti-DCIR antibody, clone 9E8 (Cat # RAB03404).
Immunofluorescence analysis of paraformaldehyde human peripheral blood monocytes fixed on Shi-fix™ coverslips, stained with the chimeric r version of RAB03404 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/mL), showing cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). The isotype control was stained with an anti-Fluorescein antibody.Flow Cytometry
Flow cytometric analysis of human peripheral blood leukocytes with CLEC4A recombinant monoclonal antibody, clone 9E8 (Cat # RAB03404).
Paraformaldehyde fixed Human peripheral blood leukocytes were stained with anti-Fluorescein IgG antibody (isotype control-black line) or the r1 version of RAB03404 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — CLEC4A
Entrez GeneID
50856Gene Name
CLEC4A
Gene Alias
CLECSF6, DCIR, DDB27, HDCGC13P, LLIR
Gene Description
C-type lectin domain family 4, member A
Omim ID
605306Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may play a role in inflammatory and immune response. Multiple transcript variants encoding distinct isoforms have been identified for this gene. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq
Other Designations
C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 6|dendritic cell immunoreceptor|lectin-like immunoreceptor
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