Ctla4 recombinant monoclonal antibody, clone 9D9
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More Files
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Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Ctla4 recombinant monoclonal antibody, clone 9D9 (Cat # RAB03394).
Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of RAB03394 at 10 ug/mL followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-Fluorescein antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03394
(B) DAPI
(C) Merged channels
(D) Isotype control
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Ctla4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against mouse Ctla4.
Reactivity
Mouse
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Blocking
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Ctla4 recombinant monoclonal antibody, clone 9D9 (Cat # RAB03394).
Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of RAB03394 at 10 ug/mL followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-Fluorescein antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03394
(B) DAPI
(C) Merged channels
(D) Isotype controlBlocking
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Gene Info — Ctla4
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