CD80 recombinant monoclonal antibody, clone 16C10
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More Files
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Immunofluorescence
Immunofluorescent staining of Daudi cells with CD80 recombinant monoclonal antibody, clone 16C10 (Cat # RAB03340).
Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03340 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03340- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of Daudi cells with CD80 recombinant monoclonal antibody, clone 16C10 (Cat # RAB03340).
Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03340 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD80.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human B7.1 antigen.
Reactivity
Human
Form
Liquid
Isotype
IgG lambda
Recommend Usage
Blocking
ELISA
Flow Cytometry
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of Daudi cells with CD80 recombinant monoclonal antibody, clone 16C10 (Cat # RAB03340).
Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03340 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03340- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Enzyme-linked Immunoabsorbent Assay
Blocking
Flow Cytometry
Flow cytometric analysis of Daudi cells with CD80 recombinant monoclonal antibody, clone 16C10 (Cat # RAB03340).
Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of RAB03340 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — CD80
Entrez GeneID
941Gene Name
CD80
Gene Alias
CD28LG, CD28LG1, LAB7
Gene Description
CD80 molecule
Omim ID
112203Gene Ontology
HyperlinkGene Summary
The B-lymphocyte activation antigen B7-1 (formerly referred to as B7) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 (MIM 186760) and CTLA4 (MIM 123890) ligands of T cells.[supplied by OMIM
Other Designations
B-lymphocyte activation antigen B7|CD80 antigen|CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)|costimulatory factor CD80|costimulatory molecule variant IgV-CD80
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Interactome
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Pathway
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Disease
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