Cd40 recombinant monoclonal antibody, clone 3/23
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More Files
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Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Cd40 recombinant monoclonal antibody, clone 3/23 (Cat # RAB03263).
RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03263 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03263- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Cd40.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against murine CD40-hγ1-Fc.
Reactivity
Mouse
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Flow Cytometry
Immunofluorescence
Immunohistochemistry
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry
Immunofluorescence
Immunofluorescent staining of RAW264.7 cells with Cd40 recombinant monoclonal antibody, clone 3/23 (Cat # RAB03263).
RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of RAB03263 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03263- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.Immunoprecipitation
Flow Cytometry
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Gene Info — Cd40
Entrez GeneID
21939Gene Name
Cd40
Gene Alias
AI326936, Bp50, GP39, HIGM1, IGM, IMD3, T-BAM, TRAP, Tnfrsf5, p50
Gene Description
CD40 antigen
Gene Ontology
HyperlinkGene Summary
member 5
Other Designations
OTTMUSP00000017667|OTTMUSP00000017739|T-cell differentiation antigen|tumor necrosis factor receptor superfamily, member 5
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