CD19 recombinant monoclonal antibody, clone CAT-13.1E10
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More Files
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Immunofluorescence
Immunofluorescent staining of human peripheral blood leukocytes with CD19 recombinant monoclonal antibody, clone CAT-13.1E10 (Cat # RAB03183).
Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric r version of RAB03183 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03183- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of Daudi cells with CD19 recombinant monoclonal antibody, clone CAT-13.1E10 (Cat # RAB03183).
Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03183 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD19.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against human CD19.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Flow Cytometry
Immunofluorescence
Therapeutics
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunofluorescence
Immunofluorescent staining of human peripheral blood leukocytes with CD19 recombinant monoclonal antibody, clone CAT-13.1E10 (Cat # RAB03183).
Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric r version of RAB03183 at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom RAB03183- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.Flow Cytometry
Flow cytometric analysis of Daudi cells with CD19 recombinant monoclonal antibody, clone CAT-13.1E10 (Cat # RAB03183).
Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of RAB03183 (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — CD19
Entrez GeneID
930Gene Name
CD19
Gene Alias
B4, MGC12802
Gene Description
CD19 molecule
Omim ID
107265Gene Ontology
HyperlinkGene Summary
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq
Other Designations
B-lymphocyte antigen CD19|CD19 antigen
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Interactome
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Pathway
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Disease
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