ROCK1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:A431 whole cell lysate Lane2:NIH/3T3 whole cell lysate with ROCK1 recombinant monoclonal antibody (Cat # RAB02768) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using ROCK1 recombinant monoclonal antibody (Cat # RAB02768).Counter stained with hematoxylin.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against ROCK1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant ROCK1.
Theoretical MW (kDa)
158
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of ROCK-1 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:A431 whole cell lysate Lane2:NIH/3T3 whole cell lysate with ROCK1 recombinant monoclonal antibody (Cat # RAB02768) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using ROCK1 recombinant monoclonal antibody (Cat # RAB02768).Counter stained with hematoxylin.Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — ROCK1
Entrez GeneID
6093Protein Accession#
Q13464Gene Name
ROCK1
Gene Alias
MGC131603, MGC43611, P160ROCK, PRO0435
Gene Description
Rho-associated, coiled-coil containing protein kinase 1
Omim ID
601702Gene Ontology
HyperlinkGene Summary
This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq
Other Designations
OTTHUMP00000162514|p160-ROCK
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Interactome
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Pathway
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Disease
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