ICAM1 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against ICAM1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant ICAM1.
Theoretical MW (kDa)
58, 96
Reactivity
Human
Specificity
This antibody detects endogenous levels of ICAM-1 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:HUVEC whole cell lysate Lane3:Raji whole cell lysate with ICAM1 recombinant monoclonal antibody (Cat # RAB02765) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ICAM1 recombinant monoclonal antibody (Cat # RAB02765). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HUVEC cells using ICAM1 recombinant monoclonal antibody (Cat # RAB02765)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — ICAM1
Entrez GeneID
3383Protein Accession#
P05362Gene Name
ICAM1
Gene Alias
BB2, CD54, P3.58
Gene Description
intercellular adhesion molecule 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq
Other Designations
cell surface glycoprotein P3.58|human rhinovirus receptor|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
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