CASP3 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:Jurkat whole cell lysate Lane3:293 whole cell lysate with CASP3 recombinant monoclonal antibody (Cat # RAB02708) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CASP3 recombinant monoclonal antibody (Cat # RAB02708). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of HeLa cells using CASP3 recombinant monoclonal antibody (Cat # RAB02708)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against CASP3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant CASP3.
Theoretical MW (kDa)
35
Reactivity
Human, Rat
Specificity
This antibody detects endogenous levels of Caspase-3 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:20-1:50)
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:Jurkat whole cell lysate Lane3:293 whole cell lysate with CASP3 recombinant monoclonal antibody (Cat # RAB02708) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CASP3 recombinant monoclonal antibody (Cat # RAB02708). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HeLa cells using CASP3 recombinant monoclonal antibody (Cat # RAB02708)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — CASP3
Entrez GeneID
836Protein Accession#
P42574Gene Name
CASP3
Gene Alias
CPP32, CPP32B, SCA-1
Gene Description
caspase 3, apoptosis-related cysteine peptidase
Omim ID
600636Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq
Other Designations
OTTHUMP00000165054|PARP cleavage protease|SREBP cleavage activity 1|Yama|apopain|caspase 3|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3
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Interactome
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Pathway
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Disease
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