H2AC4 (Hydroxyl Y39) recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:NIH/3T3 whole cell lysate with H2AC4 (Hydroxyl Y39) recombinant monoclonal antibody (Cat # RAB02704) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using H2AC4 (Hydroxyl Y39) recombinant monoclonal antibody (Cat # RAB02704). Counter stained with hematoxylin.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against H2AC4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant H2AC4.
Theoretical MW (kDa)
15
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of Histone H2A protein only when Hydroxyl at Tyr9.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:NIH/3T3 whole cell lysate with H2AC4 (Hydroxyl Y39) recombinant monoclonal antibody (Cat # RAB02704) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using H2AC4 (Hydroxyl Y39) recombinant monoclonal antibody (Cat # RAB02704). Counter stained with hematoxylin. -
Gene Info — HIST1H2AB
Entrez GeneID
8335Protein Accession#
P04908;P28001;35065;Q93077;Q99878Gene Name
HIST1H2AB
Gene Alias
H2A/m, H2AFM
Gene Description
histone cluster 1, H2ab
Omim ID
602795Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq
Other Designations
H2A histone family, member M|OTTHUMP00000016137|histone 1, H2ab
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Interactome
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Pathway
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Disease
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