HSPA8 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:HepG2 whole cell lysate Lane2:Hela whole cell lysate Lane3:NIH/3T3 whole cell lysate Lane4:PC12 whole cell lysate with HSPA8 recombinant monoclonal antibody (Cat # RAB02702) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using HSPA8 recombinant monoclonal antibody (Cat # RAB02702).Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells using HSPA8 recombinant monoclonal antibody (Cat # RAB02702)(green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against HSPA8.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant HSPA8.
Theoretical MW (kDa)
71
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of HSC 70 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:HepG2 whole cell lysate Lane2:Hela whole cell lysate Lane3:NIH/3T3 whole cell lysate Lane4:PC12 whole cell lysate with HSPA8 recombinant monoclonal antibody (Cat # RAB02702) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using HSPA8 recombinant monoclonal antibody (Cat # RAB02702).Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells using HSPA8 recombinant monoclonal antibody (Cat # RAB02702)(green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
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Gene Info — HSPA8
Entrez GeneID
3312Protein Accession#
P11142Gene Name
HSPA8
Gene Alias
HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP1, MGC131511, MGC29929, NIP71
Gene Description
heat shock 70kDa protein 8
Omim ID
600816Gene Ontology
HyperlinkGene Summary
The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq
Other Designations
LPS-associated protein 1|N-myristoyltransferase inhibitor protein 71|constitutive heat shock protein 70|heat shock 70kD protein 8|heat shock 70kd protein 10|heat shock cognate protein 54|heat shock cognate protein, 71-kDa|lipopolysaccharide-associated pro
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Interactome
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Pathway
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Disease
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