PHB1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane1:293 whole cell lysate Lane2:Jurkat whole cell lysate Lane3:The kidney tissue lysate of Mouse Lane4:HepG2 whole cell lysate with PHB1 recombinant monoclonal antibody (Cat # RAB02700) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using PHB1 recombinant monoclonal antibody (Cat # RAB02700). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells using PHB1 recombinant monoclonal antibody (Cat # RAB02700)(green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
Flow Cytometry
Flow cytometric analysis of HepG2 cells with PHB1 recombinant monoclonal antibody (Cat # RAB02700) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against PHB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant PHB1.
Theoretical MW (kDa)
30
Reactivity
Human, Mouse, Rat, Zebra fish
Specificity
This antibody detects endogenous levels of PHB and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunohistochemistry (1:200-1:500)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1:293 whole cell lysate Lane2:Jurkat whole cell lysate Lane3:The kidney tissue lysate of Mouse Lane4:HepG2 whole cell lysate with PHB1 recombinant monoclonal antibody (Cat # RAB02700) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using PHB1 recombinant monoclonal antibody (Cat # RAB02700). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of NIH/3T3 cells using PHB1 recombinant monoclonal antibody (Cat # RAB02700)(green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Flow Cytometry
Flow cytometric analysis of HepG2 cells with PHB1 recombinant monoclonal antibody (Cat # RAB02700) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. -
Gene Info — PHB
Entrez GeneID
5245Protein Accession#
P35232Gene Name
PHB
Gene Alias
PHB1
Gene Description
prohibitin
Omim ID
176705Gene Ontology
HyperlinkGene Summary
Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq
Other Designations
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Interactome
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Disease
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