RHOC recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against RHOC.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant RHOC.
Theoretical MW (kDa)
24
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of Rho A/B/C and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:200-1:500)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:HepG2 whole cell lysate Lane2:Hela whole cell lysate Lane3:Jurkat whole cell lysate with RHOC recombinant monoclonal antibody (Cat # RAB02692) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using RHOC recombinant monoclonal antibody (Cat # RAB02692). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of Ags cells using RHOC recombinant monoclonal antibody (Cat # RAB02692)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — RHOC
Entrez GeneID
389Protein Accession#
P08134Gene Name
RHOC
Gene Alias
ARH9, ARHC, H9, MGC1448, MGC61427, RHOH9
Gene Description
ras homolog gene family, member C
Omim ID
165380Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000013675|OTTHUMP00000013676|OTTHUMP00000013802|OTTHUMP00000013805|OTTHUMP00000013807|OTTHUMP00000013809|RAS-related homolog 9|Rho-related GTP-binding protein RhoC|oncogene RHO H9|rhoC GTPase|small GTP binding protein RhoC
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Interactome
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Disease
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