NGFR recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Lane1:The skeletal muscle tissue lysate of Human with NGFR recombinant monoclonal antibody (Cat # RAB02665) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human uterus tissue using NGFR recombinant monoclonal antibody (Cat # RAB02665). Counter stained with hematoxylin
Immunocytochemistry
Immunocytochemical staining of HeLa cells using NGFR recombinant monoclonal antibody (Cat # RAB02665)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against NGFR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant NGFR.
Theoretical MW (kDa)
45, 75
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of NGFR p75 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of Lane1:The skeletal muscle tissue lysate of Human with NGFR recombinant monoclonal antibody (Cat # RAB02665) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human uterus tissue using NGFR recombinant monoclonal antibody (Cat # RAB02665). Counter stained with hematoxylinImmunocytochemistry
Immunocytochemical staining of HeLa cells using NGFR recombinant monoclonal antibody (Cat # RAB02665)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Immunoprecipitation
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Gene Info — NGFR
Entrez GeneID
4804Protein Accession#
P08138Gene Name
NGFR
Gene Alias
CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR
Gene Description
nerve growth factor receptor (TNFR superfamily, member 16)
Omim ID
162010Gene Ontology
HyperlinkGene Summary
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq
Other Designations
low affinity nerve growth factor receptor|low-affinity nerve growth factor receptor|nerve growth factor receptor
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Interactome
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Pathway
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Disease
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