LRP1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane1:The liver tissue lysate of Mouse Lane2:The brain tissue lysate of Mouse Lane3:The lung tissue lysate of Mouse Lane4:The liver tissue lysate of Human Lane5:HepG2 whole cell lysate Lane6:The lung tissue lysate of Human with LRP1 recombinant monoclonal antibody (Cat # RAB02655) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung tissue using LRP1 recombinant monoclonal antibody (Cat # RAB02655). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of HUVEC cells using LRP1 recombinant monoclonal antibody (Cat # RAB02655). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
Flow Cytometry
Flow cytometric analysis of Hela cells with LRP1 recombinant monoclonal antibody (Cat # RAB02655) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against LRP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant LRP1.
Theoretical MW (kDa)
85
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of LRP1 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:10-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane1:The liver tissue lysate of Mouse Lane2:The brain tissue lysate of Mouse Lane3:The lung tissue lysate of Mouse Lane4:The liver tissue lysate of Human Lane5:HepG2 whole cell lysate Lane6:The lung tissue lysate of Human with LRP1 recombinant monoclonal antibody (Cat # RAB02655) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung tissue using LRP1 recombinant monoclonal antibody (Cat # RAB02655). Counter stained with hematoxylin.Immunohistochemistry
Immunocytochemistry
Immunocytochemical staining of HUVEC cells using LRP1 recombinant monoclonal antibody (Cat # RAB02655). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
Flow cytometric analysis of Hela cells with LRP1 recombinant monoclonal antibody (Cat # RAB02655) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. -
Gene Info — LRP1
Entrez GeneID
4035Protein Accession#
Q07954Gene Name
LRP1
Gene Alias
A2MR, APOER, APR, CD91, FLJ16451, IGFBP3R, LRP, MGC88725, TGFBR5
Gene Description
low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor)
Omim ID
107770Gene Ontology
HyperlinkOther Designations
TbetaR-V/LRP-1/IGFBP-3 receptor|alpha-2-macroglobulin receptor|low density lipoprotein-related protein 1|type V tgf-beta receptor
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Interactome
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Disease
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