TJP1 recombinant monoclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using TJP1 recombinant monoclonal antibody (Cat # RAB02654). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of MCF7 cells using TJP1 recombinant monoclonal antibody (Cat # RAB02654). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against TJP1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide (KLH-coupled) corresponding to TJP1.
Theoretical MW (kDa)
194
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of ZO1 and does not cross-react with related proteins.
Form
Liquid
Purification
Peptide affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:200)
Immunohistochemistry (1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using TJP1 recombinant monoclonal antibody (Cat # RAB02654). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of MCF7 cells using TJP1 recombinant monoclonal antibody (Cat # RAB02654). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS. -
Gene Info — TJP1
Entrez GeneID
7082Protein Accession#
Q07157Gene Name
TJP1
Gene Alias
DKFZp686M05161, MGC133289, ZO-1
Gene Description
tight junction protein 1 (zona occludens 1)
Omim ID
601009Gene Ontology
HyperlinkGene Summary
This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174520|tight junction protein 1|tight junction protein ZO-1|zona occludens 1|zonula occludens 1 protein
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Interactome
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Pathway
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