THBS1 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human THBS1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein of human THBS1.
Theoretical MW (kDa)
160
Reactivity
Human, Mouse
Specificity
Recognizes endogenous levels of Thrombospondin 1 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MCF7 (A), 293T (B), C2C12 (C) whole cell lysates with THBS1 recombinant monoclonal antibody (Cat # RAB02614).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human cholangiocarcinoma formalin fixed paraffin embedded tissue section using THBS1 recombinant monoclonal antibody (Cat # RAB02614). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.49). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
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Gene Info — THBS1
Entrez GeneID
7057Protein Accession#
P07996Gene Name
THBS1
Gene Alias
THBS, TSP, TSP1
Gene Description
thrombospondin 1
Omim ID
188060Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq
Other Designations
thrombospondin-1p180
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Interactome
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