COL1A1 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human COL1A1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein of human COL1A1.
Theoretical MW (kDa)
160
Reactivity
Human
Specificity
Recognizes endogenous levels of Collagen 1 alpha 1 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A549 (A), HL60 (B), U2OS (C) whole cell lysates with COL1A1 recombinant monoclonal antibody (Cat # RAB02583).Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HeLa cells with COL1A1 recombinant monoclonal antibody (Cat # RAB02583). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.Immunoprecipitation
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Gene Info — COL1A1
Entrez GeneID
1277Protein Accession#
P02452Gene Name
COL1A1
Gene Alias
OI4
Gene Description
collagen, type I, alpha 1
Gene Ontology
HyperlinkGene Summary
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish
Other Designations
alpha 1 type I collagen|collagen alpha 1 chain type I|collagen of skin, tendon and bone, alpha-1 chain|pro-alpha-1 collagen type 1|type I collagen alpha 1 chain
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Interactome
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