BCR recombinant monoclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A549 (A), HL60 (B), U2OS (C), C6 (D) whole cell lysates with BCR recombinant monoclonal antibody (Cat # RAB02577).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human brain formalin fixed paraffin embedded tissue section using BCR recombinant monoclonal antibody (Cat # RAB02577). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.1). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human BCR.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human BCR.
Theoretical MW (kDa)
160
Reactivity
Human, Rat
Specificity
Recognizes endogenous levels of BCR protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:10-1:50)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of A549 (A), HL60 (B), U2OS (C), C6 (D) whole cell lysates with BCR recombinant monoclonal antibody (Cat # RAB02577).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human brain formalin fixed paraffin embedded tissue section using BCR recombinant monoclonal antibody (Cat # RAB02577). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.1). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunoprecipitation
-
Gene Info — BCR
Entrez GeneID
613Protein Accession#
P11274Gene Name
BCR
Gene Alias
ALL, BCR-ABL1, BCR1, CML, D22S11, D22S662, FLJ16453, PHL
Gene Description
breakpoint cluster region
Gene Ontology
HyperlinkGene Summary
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
bcr-abl1 e14a3 chimeric protein
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com