WDR1 recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Jurkat (A) whole cell lysates with WDR1 recombinant monoclonal antibody (Cat # RAB02558).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human cholangiocarcinoma formalin fixed paraffin embedded tissue section using WDR1 recombinant monoclonal antibody (Cat # RAB02558). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.46). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescence
Immunofluorescent analysis of HeLa cells with WDR1 recombinant monoclonal antibody (Cat # RAB02558). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human WDR1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide of human WDR1.
Theoretical MW (kDa)
66
Reactivity
Human
Specificity
Recognizes endogenous levels of WDR1 protein.
Form
Liquid
Purification
Immunogen affinity chromatography
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)Storage Buffer
In 50mM Tris-Glycine, pH 7.4 (0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Jurkat (A) whole cell lysates with WDR1 recombinant monoclonal antibody (Cat # RAB02558).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of human cholangiocarcinoma formalin fixed paraffin embedded tissue section using WDR1 recombinant monoclonal antibody (Cat # RAB02558). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.46). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.Immunocytochemistry
Immunofluorescence
Immunofluorescent analysis of HeLa cells with WDR1 recombinant monoclonal antibody (Cat # RAB02558). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. -
Gene Info — WDR1
Entrez GeneID
9948Protein Accession#
O75083Gene Name
WDR1
Gene Alias
AIP1, NORI-1
Gene Description
WD repeat domain 1
Omim ID
604734Gene Ontology
HyperlinkGene Summary
This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
WD repeat-containing protein 1|actin interacting protein 1
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